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Features

- Next generation method improves PCR inhibitor removal efficiency
- Processes all soil types, including difficult environmental samples
- Patented technology removes 100% of humic substances and PCR inhibitors
- Rapid protocol enables purification of DNA from 250 mg soil in 30 minutes
- Applications include biodefense, soil-borne pathogen detection, agriculture and zoology research

Description

The PowerSoil® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.

The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.

Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.

NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!

Research and Development

The PowerSoil® DNA Isolation Kit is More Effective for Community Analysis by PCR

High Yield, Intact Genomic DNA

Total Genomic DNA was isolated from nine different sample types using the MO BIO PowerSoil® DNA Isolation Kit and soil DNA isolation kits from two other suppliers. All isolations were performed following the manufacturer¨ªs protocols. Eluted genomic DNA was displayed on a 0.8% TAE agarose gel (15 ¦Ìl per lane).

Confidence in Your PCR Analysis

PCR analysis using eubacterial primers was performed on 1 ¦Ìl of the undiluted DNA eluate. PCR products were displayed on a 0.8% TAE agarose gel.

Lane M is DNA Marker.

Lane 1 - Landfill 0-3 inches				Lane 4 - Coffee Compost				Lane 7 - Mud Sediment
Lane 2 - Landfill 3-6 inches				Lane 5 - Marine Sediment				Lane 8 - Horse Manure
Lane 3 - Late-stage Compost				Lane 6 - Lake Sediment				Lane 9 - Mulch Topsoil

Positive PCR amplification using PowerSoil® was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with supplier kits tested.

Specifications

Format	Silica Spin Filter Tubes
Method	Bead Beating
Binding Capacity	Up to 20 ¦Ìg per filter
Sample Size	250 mg
Throughput	1 - 24 samples
Time	30 minutes
Equipment Required	Vortex and Vortex Adapter

Storage Conditions

Store at room temperature for up to 2 years.

Publications

• Alcantara-Hernandez, R.J., Valenzuela-Encinas, C., Marsch, R., and Dendooven, L. Respiratory and Dissimilatory Nitrate-reducing Communities from and Extreme Saline Alkaline Soil of the Former Lake Texcoco (Mexico). Extremophiles. 13(1): 169-178 (2009).
• Foster, J.S., Green, S.J., Ahrendt, S.R., Golubic, S., Reid, R.P., Jetherington, K.L., and Bebout, L. Molecular and Morphological Characterization of Cyanobacterial Diversity in the Stormatolites of Highborne Cay, Bahamas. ISME Journal. 1-15 (2009).
• Giubilei, M.A., Leonardi, V., Federici, E., Covino, S., Sasek, V., Novotny, C, Federici, F., D¡¯Annibale, A., and Petruccioli, M. Effect of Mobilizing Agents on Mycoremediation and Impact on the Indigenous Microbiota. Chemical Technology and Biotechnology. 84(6): 836-844 (2009).

Kit Components

Component	12888-50
PowerBead Tubes	50
Solution C1	1 x 3.3 ml
Solution C2	1 x 14 ml
Solution C3	1 x 11 ml
Solution C4	1 x 72 ml
Solution C5	1 x 27.5 ml
Solution C6	1 x 6 ml
Spin Filters In Tubes	50
Collection Tubes	200