PowerSoil DNA / UltraClean Soil DNA / PowerMax Soil DNA / UltraClean Soil Mega DNA
The PowerSoil® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.
The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.
Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.
NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!
The PowerSoil® DNA Isolation Kit is More Effective for Community Analysis by PCR
Total Genomic DNA was isolated from nine different sample types using the MO BIO PowerSoil® DNA Isolation Kit and soil DNA isolation kits from two other suppliers. All isolations were performed following the manufacturer¨ªs protocols. Eluted genomic DNA was displayed on a 0.8% TAE agarose gel (15 ¦Ìl per lane).
PCR analysis using eubacterial primers was performed on 1 ¦Ìl of the undiluted DNA eluate. PCR products were displayed on a 0.8% TAE agarose gel.
Lane M is DNA Marker.
Lane 1 - Landfill 0-3 inches | Lane 4 - Coffee Compost | Lane 7 - Mud Sediment | ||||||
Lane 2 - Landfill 3-6 inches | Lane 5 - Marine Sediment | Lane 8 - Horse Manure | ||||||
Lane 3 - Late-stage Compost | Lane 6 - Lake Sediment | Lane 9 - Mulch Topsoil |
Positive PCR amplification using PowerSoil® was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with supplier kits tested.
Format | Silica Spin Filter Tubes |
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Method | Bead Beating |
Binding Capacity | Up to 20 ¦Ìg per filter |
Sample Size | 250 mg |
Throughput | 1 - 24 samples |
Time | 30 minutes |
Equipment Required | Vortex and Vortex Adapter |
Component | 12888-50 |
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PowerBead Tubes | 50 |
Solution C1 | 1 x 3.3 ml |
Solution C2 | 1 x 14 ml |
Solution C3 | 1 x 11 ml |
Solution C4 | 1 x 72 ml |
Solution C5 | 1 x 27.5 ml |
Solution C6 | 1 x 6 ml |
Spin Filters In Tubes | 50 |
Collection Tubes | 200 |