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PowerSoil® DNA Isolation Kit
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UltraClean® Mega Soil DNA Isolation Kit

Features

- Established method selectively removes most PCR inhibitors
- Rapidly purify DNA from up to one gram of soil in 30 minutes
- Yields high quality PCR ready DNA up to 50 kb plus
- Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR
- Efficiently lyses spores and fungi; also compatible with fecal samples

Description

The UltraClean® Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.

Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer.

This is our original DNA kit for soil - for more challenging soil samples we recommend the PowerSoil® DNA Isolation Kit

NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!

Research and Development

Purification of DNA from various soils using the UltraClean® Soil DNA Isolation Kit

Lane 1: Forest soil
Lane 2: Bog soil
Lane 3: Pristine sand
Lane 4: Farm soil

PCR amplification of DNA extracted with this soil kit using universal eubacterial primers

Lane 1: Marker
Lane 2: Negative control
Lanes 3-8: Six different soil types

Learn more about the applications of the UltraClean® Soil DNA Isolation Kit in this poster presented at the American Society for Microbiology Meeting.

Specifications

Format	Silica Spin Filter Tubes
Method	Bead Beating
Binding Capacity	Up to 20 ¦Ìg per filter
Throughput	1 - 24 samples
Time	30 minutes
Equipment Required	Vortex Adapter

Positive PCR Results Obtained with these Soil Types
Clay Fecal/Intestinal Tract Fungal Mat Forest	Loam Sand Potting Soil Petroleum Saturated

Storage Conditions

Store at room temperature for up to 2 years.

Publications

• Andreote, F., Carneiro, R., Salles, J., Marcon, J., Labate, C., Azevedo, J., and Araujo, W. Culture-Independent Assessment of Rhizobiales-Related Alphaproteobacteria and the Diversity of Methylobacterium in the Rhizosphere and Rhizoplane of Transgenic Eucalyptus. Microbial Ecology. 57: 82-93 (2009).
• Baniulyte, D., Favila, E., and Kelly, J.Shifts in Microbial Community Composition Following Surface Application of Dredged River Sediment. Microbial Ecology. 57: 160-169 (2009).
• Barkovskii, A., Fukui, H., Leisen, J., Kim, S., Marsh, T., and Khijniak, A.Rearrangement of Bacterial Community Structure During Peat Diagenesis.Soil Biology and Biochemistry. 41: 135-143 (2009).

Customer Comments

I consulted a soil microbiologist for advice on a protocol for rapid DNA extraction from soil samples. He recommended the MO BIO UltraClean^® Soil DNA Isolation Kit. My research has involved extensive soil sampling. The soil is analyzed for the presence of Streptomyces scabies, causal agent of potato scab, which is detectable by PCR. I have used the kit to process samples and have been extremely pleased with the results of not only getting consistent amounts of DNA, but also DNA that is high quality for PCR. I will continue using this kit because it is consistent and extremely quick. No other kit I have used is even half as fast.
 

Elise C. Poole
Michigan State University
Dept. of Botany and Plant Pathology

Read More Customer Comments
on the UltraClean^® Soil DNA Kit

Kit Components

Component	12888-50
PowerBead Tubes	50
Solution C1	1 x 3.3 ml
Solution C2	1 x 14 ml
Solution C3	1 x 11 ml
Solution C4	1 x 72 ml
Solution C5	1 x 27.5 ml
Solution C6	1 x 6 ml
Spin Filters In Tubes	50
Collection Tubes	200