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Vortex Adapters for Vortex-Genie® 2
PowerMax® Soil DNA Isolation Kit
UltraClean® Soil DNA Isolation Kit

Features

- Purifies DNA from 5 to 10 grams of soil in 50 minutes
- Yields PCR ready DNA up to 50 kb plus
- Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR
- Efficiently lyses spores and fungi and fecal samples - Ideal for samples with low microbial load
- All tubes and reagents required are included in kit and ready to use

Description

The UltraClean® Mega Soil DNA Isolation Kit provides researchers the ability to process larger volumes of soil, critical when microbial load is low. This reliable kit is based on a bead beating technology that offers a proprietary Inhibitor Removal Solution to remove PCR inhibitors including humic acid.The UltraClean® Mega Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors. Our 50 ml Vortex Adapter for cell lysis and a centrifuge capable of spinning 50 ml tubes are required for maximum performance.

Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer.

This is our original mega soil DNA kit - for more challenging 10 g soil samples we recommend the PowerMax® Soil DNA Isolation Kit.

Research and Development

Purification of DNA from various soils using
the UltraClean® Mega Soil DNA Isolation Kit

Lane 1: Forest soil;
Lane 2: Bog soil;
Lane 3: Pristine sand;
Lane 4: Farm soil

PCR amplification of DNA extracted with this soil kit using universal eubacterial primers

Lane 1: Marker
Lane 2: Negative control
Lanes 3-8: Six different soil types

Specifications

Format	Silica Spin Filter Tubes
Method	Bead Beating
Sample Size	Up to 10 grams
Binding Capacity	Up to 1mg per filter
Throughput	1 - 8 samples
Time	50 minutes
Equipment Required	Vortex and Centrifuge

Positive PCR Results Obtained with these Soil Types
Clay Fecal/Intestinal Tract Fungal mat Forest	Loam Sand Potting Soil Petroleum Saturated

Storage Conditions

Store at room temperature for up to 2 years.

Publications

• Nunoura, T. and Takai, K. Comparison of Microbial Communities Associated with Phase-separation-induced Hydrothermal Fluids at the Yonaguni Knoll IV Hydrothermal Field, the Southern Okinawa Trough. FEMS Microbiology Ecology. 67(3): 351-370 (2009).
• Nunoura, T., Inagaki, F., Delwiche, M.E., Colwell, F.S., and Takai, K. Subseafloor Microbial Communities in Methane Hydrate-bearing Sediment at Two Distinct Locations (ODP Leg204) in the Cascadia Margin. Microbes and Environment. 23(4): 317-328 (2008).
• Fell, J.W., Scorzetti, G., Connell, L., and Craig, S. Biodiversity of Micro-eukaryotes in Antarctic Dry Valley Soils with <5% Soil Moisture. Soil Biology and Biochemistry. 38(10): 3107-3119 (2006).

Customer Comments

UltraClea® Mega Soil DNA Isolation Kit

I consulted a soil microbiologist for advice on a protocol for rapid DNA extraction from soil samples. He recommended the MO BIO UltraClean® Mega Soil DNA Isolation Kit. My research has involved extensive soil sampling. The soil is analyzed for the presence of Streptomyces scabies, causal agent of potato scab, which is detectable by PCR. I have used the kit to process samples and have been extremely pleased with the results of not only getting consistent amounts of DNA, but also DNA that is high quality for PCR. I will continue using this kit because it is consistent and extremely quick. No other kit I have used is even half as fast.

Elise C. Poole
Michigan State University
Dept. of Botany and Plant Pathology

Kit Components

Component	12900-10
Mega Bead Tubes Bead Solution Solution S1 IRS Solution Solution S2 Solution S3 Solution S4 Solution S5 Spin Filters In Tubes Collection Tubes	10 1 x 165 ml 1 x 14 ml 1 x 44 ml 1 x 22 ml 1 x 330 ml 2 x 30 ml 1 x 88 ml 10 30